Strand-specific, high-resolution mapping of modified RNA polymerase II

Tollervey lab paper featured in Molecular Systems Biology.

Image
Image from Tollervey paper, Molecular Systems Biology 2016

Authors

Milligan, L., Huynh-Thu, V.A., Delan-Forino, C., Tuck, A., Petfalski, E., Lombraña, R., Sanguinetti, G., Kudla, G. and Tollervey, D.

Summary

A new technique for transcriptome‐wide mapping of RNAPII carrying the five types of CTD phosphorylation is presented. Distinct modification states associated with initiating, early and late elongating RNAPII are identified using a hidden Markov model.

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