Cell-specific and lamin-dependent targeting of novel transmembrane proteins in the nuclear envelope

Schirmer lab paper featured in Cellular and Molecular Life Sciences.

Image
Image from Schirmer paper, Cellular and Molecular Life Sciences 2010

Authors

Malik P, Korfali N, Srsen V, Lazou V, Batrakou DG, Zuleger N, Kavanagh DM, Wilkie GS, Goldberg MW, Schirmer EC.

Cell Mol Life Sci. 2010 Jan 21. [Epub ahead of print]

Summary

In Malik et al., the Schirmer lab used the recently developed high resolution structured illumination microscopy (OMX) funded by SULSA to distinguish whether several novel nuclear envelope transmembrane (NET) proteins they discovered are in the inner or outer nuclear membrane.  If the NET is in the inner nuclear membrane it should be in the same plane as  Nup153 on the nucleus side of the nuclear pore complex and in an inner plane compared to Nup358 on the cytoplasmic side.  Regular light microscopy is not capable of such high resolution because only ~85 nm separates Nup153 from Nup358

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