Sawin lab paper featured in Molecular & Cellular Proteomics. Image Changes in fission yeast protein levels during progression from late G2 to G1/S. log2 protein expression ratios (G1/S versus late G2) for 2964 fission yeast proteins (x axis) plotted against the sum of the relevant peptide intensities (y axis). Authors Bicho C., de Lima F. Chen Z, Rappsilber J and Sawin KE Summary The Sawin/Rappsilber labs have developed a new strategy to avoid a problem associated with SILAC analysis: the in vivo conversion of labeled arginine to other amino acids. Bicho and coauthors avoided this problem using a simple but efficient genetic engineering trick (the deletion of genes involved in the catabolism of arginine) and a slight modification of the growing medium of the yeast mutant strains. The figure shows how this new fission yeast SILAC methodology can be applied successfully to the identification of both proteins that were up-regulated and proteins that were down-regulated as cells progressed from G2 to G1/S. Related links DOI Journal Link This article was published on 2024-06-17
