Allshire lab paper featured in Nature Structural and Molecular Biology. Image Authors Matthew D D Miell, Colin J Fuller, Annika Guse, Helena M Barysz, Andrew Downes, Tom Owen-Hughes, Juri Rappsilber, Aaron F Straight & Robin C Allshire Summary Nucleosomes with histone H3 replaced by CENP-A direct kinetochore assembly. CENP-A nucleosomes from human and Drosophila have been reported to have reduced heights as compared to canonical octameric H3 nucleosomes, thus suggesting a unique tetrameric hemisomal composition. We demonstrate that octameric CENP-A nucleosomes assembled in vitro exhibit reduced heights, indicating that they are physically distinct from H3 nucleosomes and negating the need to invoke the presence of hemisomes. Related links Doi Journal Link This article was published on 2024-06-17
