Information about the Leica Sp5. Available Lasers Laser Line Power at x20 objective 405nm 0.7mW 458nm NA 488nm 10% of maximum power 0.8mW 514nm 10% of maximum power 0.3mW 561nm 1.6mW 594nm 0.3mW 633nm 2.1mW Image Software Leica LAS-AF (Confocal) ISS Vista (FCS) Available Modules Conventional and Resonant Scanners 5 detection channels (2xHyD) Spectrometers (all visible wavelengths) FRAP, FRET FCS,FCCS 8K x 8K image size on conventional scanner 25 frames/sec at 512x512 using resonant scanner Objectives Type Magnification Correction NA HCX PLAN APO x20 Oil, Water, Lbd Bl 0.7 HCX PLAPO x40 Oil, PH3, CS 1.25 HCX PLAPO x63 W Corr Lbd Bl 1.2 HCX PLAPO x63 Glycerol 1.3 HCX PLAPO x63 Oil CS 1.4 HCX PLAPO x100 Oil 1.46 Supporting Equipment Environmental chamber Temperature control from ambient to 45°C Humidified CO2 concentration 1% to 11% Fluorescence Correlation Spectroscopy (FCS) Single pixel beam positioning Dual channel for Fluorescence Cross Correlation Spectroscopy (FCCS) 3 Filter Sets available GFP/RFP - Band Pass filters 500-550nm and 607-683nm, Beam Splitter 560nm CFP/YFP - Band Pass filters 470-500nm and 535-585nm, Beam Splitter 505nm Alexa 488/633 - Band Pass filters 500-550nm and 647-703nm, Beam Splitter 625nm Room Location Swann 2.15 This article was published on 2024-06-17