Information about Zeiss Axio Observer equipment. Filter Sets Fluorophore Excitation Dichroic Emitter Filter Set DAPI UV: 385/30nm 405 395 425/30 445/50 Zeiss Set 90 HE LED Zeiss Set 49 CFP V: 423/44nm 450 467/24 Zeiss Set 91 HE LED GFP/FITC/Alexa 488 B: 469/38nm 495 493 525/50 514/30 Zeiss Set 38 HE Zeiss Set 90HE LED YFP C: 511/44nm 538 555/25 Zeiss Set 91 LED RFP/tdTomato G: 555/30nm 570 575 605/70 592/25 Zeiss Set 43 HE Zeiss Set 90 HE LED mCherry Y: 590/27nm 610 687/185 Zeiss Set 91 LED Cy 5 R: 631/33nm 653 709/100 Zeiss Set 90 HE LED Image Software Zen 2.3 Available Modules 4D Acquisition Triggered Acquisition for high speed applications Scanning Stage for multipoint acquisitions Hamamatsu Orca Flash 4 V2 Definite Focus II Objectives Type Magnification Correction NA Plan ApoChromat x40 Oil, DIC 1.4 Plan ApoChromat x60 Oil, DIC 1.4 alpha-Plan ApoChromat x100 Oil, DIC 1.46 Supporting Equipment Environmental Chamber Temperature Control Ambient to 45°C Humidified CO2 concentration 1% to 10% Room Location Swann 2.10 This article was published on 2024-06-17
