Zeiss LSM 980 (Airyscan 2)

Information about Zeiss LSM 980 (Airyscan 2) equipment.

Available Lasers

LaserLaser Power at x20 Objective
405nm4.8mW
488nm6.2mW
561nm6.8mW
594nm1.6mW
633nm5.5mW

Software

  • Zen Blue 3.3 (on microscope)
  • Zen Black 2.3 (Off line)
  • Zen Blue 3.3 (Off Line)

Available Modules

  • Fluorescence Recovery after Photobleaching (FRAP)
  • Fluorescence Correlation Spectroscopy(FCS)
  • 4D imaging (Time,X,Y and Z)
  • Definite Focus
  • Colocalisation
  • Spectral Unmixing
  • Tiles & Positions
  • XY scanning stage
  • 2 x PMT's detectors
  • 1 x GaAsp detector
  • Airyscan detector (120nm XY and 350nm Z)
Photo of Zeiss LSM 980 Airyscan 2

Objectives

TypeMagnificationCorrectionNAContrast Method
Plan Apochromatx20Air0.8 
Plan Apochromatx63Water1.2 
Plan Apochromatx63Oil1.4DIC
Alpha Plan Apochromatx100Oil1.45DIC

AiryScan 2 (with 4Y 25 frames per second)

Suggested Laser LinesEmission Filter
405nm  488nmBP420-480  BP495-550
405nm  561nmBP420-480  BP570-630
405nm  561nm  594nmBP420-500  LP605
488nm  561nm  594nmBP495-555  BP570-630
488nm  633nmBP495-555  LP660
561nm  633nmBP570-620  LP660

Supporting Equipment

  • Environmental Chamber
  • Temperature Control Ambient - 45ºC
  • CO2 Module (1-10%)
  • Slide Mounting Frame
  • 35mm Petri dish frame
  • Well Plate frame

Room Location

March G.7

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