Zeiss LSM 990 (Airyscan)

Information about Zeiss LSM 990 with Fast Airyscan equipment.

Available Laser Lines

LineMaximum Power at x20 Objective
405nm5.6mW
488nm7.1mW
561nm8.9mW
594nm2.6mW
633nm6.0mW

Software

  • Zen Blue 2.3 (Off Line)

Available Modules

  • Fluorescence Recovery after Photobleaching (FRAP)
  • 4D imaging (Time, X, Y and Z)
  • Definite Focus
  • Colocalisation
  • Spectral Unmixing
  • Tiles & Positions
  • XY scanning stage
  • 2 x PMT's detectors
  • 1 x GaAsp detector
  • Airyscan detector (120nm XY and 350nm Z)
Photo of the Zeiss LSM 990 (Airyscan)

Objectives

TypeMagnificationCorrectionNA
Plan Apochromatx10Air0.45
Plan Apochromatx20Air0.8
Plan Apochromatx63Oil1.4
Alpha Plan Apochromatx100Oil1.46

Airyscan (with Fast module)

Suggested Laser LineEmission Filter
405nm 488nmBP 420-480 + BP 495-550
488nm, 561nmBP 495-550 + BP 570-630
405nm, 594nm, 633nmBP 420-500 + LP 605
488nm, 561nm, 594nmBP 495-550 + BP 570-630
488nm, 633nmBP 495-555 + LP 660
561nm, 594nm, 633nmBP 570-620 + LP 600

Supporting Equipment

  • Environmental Chamber
  • Temperature control (Ambient - 45°C)
  • CO2 module (1-10%)

Room Location

March G.9

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