Nikon Ti2 Widefield/CSU-W1 Spinning Disk with incubation chamber

Information about Nikon Ti2 Widefield/CSU-W1 Spinning Disk Live Imaging Microscope equipment.

Available Exciter/Emitter Combinations (Widefield)

Available Filter Sets

Suggested FluorophoreSpectraX IlluminationAvailable Excitation FilterAvailable DichroicAvailable Emission Filter
DAPI378/52377/26409432/36
CFP438/24438/24459482/24
GFP474/26474/26493525/40 or 515/30
YFP509/22509/22526544/24 or 543/22
Cy3/tdTomato554/23543/23573596/83 or 595/31
TxRed/mCherry575/25578/21596641/75 or 702/197
Far Red/Cy5635/18638/18652698/70 or 730/139
Photo of Nikon Ti2 Live Imaging Microscope

Available Exciter/Emitter Combinations (Spinning Disk)

Available Filter Sets

FluorophoreExcitation LaserEmission
DAPI405nm447/60 nm
eGFP488nm525/30nm
Cy3561nm600/52nm
Cy3561nm600/52nm
Texas Red561nm (~50% efficient)641/75 nm
Cy5633nm708/75nm

Software

Nikon Elements 5.1

Available Modules

  • 4D acquisition
  • Multi-stage position
  • Triggered acquisition
  • Perfect focus for long term timelapse
  • JOBS acquisition
  • Photometrics Prime 95B

Objectives

TypeMagnificationCorrectionNA
CFI Plan Apochromat Lambdax20Air0.75
CFI Plan Apochromat Lambdax40Air0.95
CFI Plan Apochromat Lambdax60Oil1.3
CFI Plan Apochromat TIRFx100Oil1.49

Supporting Equipment

  • OKOLab Environmental chamber
  • Active CO2 Controller (1 to 11%)
  • Temperature Controller (Ambient to 45°C)

Room Location

March G.6

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