ImageJ

ImageJ

All our Imaging Computers have ImageJ/Fiji (https://fiji.sc/) installed. This open-source software can be used for most image processing and analysis tasks and opens all proprietary file formats from our microscopes.The functionality of ImageJ can be extended through the installation of plugins and use of macros to automate pipelines. The LMC staff are available to design and write bespoke plugins to solve your image analysis problems. Example of some plugins we’ve written can be found here; https://github.com/orgs/COIL-Edinburgh/repositoriesWe frequently contribute plugins that produce data for use in publications, some recent examples;

• Mihalas, B.P., Pieper, G.H., Aboelenain, M., Munro, L., Srsen, V., Currie, C.E., Kelly, D.A., Hartshorne, G.M., Telfer, E.E., McAinsh, A.D. and Anderson, R.A., 2024. Age-dependent loss of cohesion protection in human oocytes. Current Biology, 34(1), pp.117-131.
• Pantier, R., Brown, M., Han, S., Paton, K., Meek, S., Montavon, T., Shukeir, N., McHugh, T., Kelly, D.A., Hochepied, T. and Libert, C., 2024. MeCP2 binds to methylated DNA.  Nature Communications, 15(1), p.3880.
  • Parashara P, Medina-Pritchard B, Abad MA, Sotelo-Parrilla P, Thamkachy R, Grundei D, Zou J, Spanos C, Kumar CN, Basquin C, Das V, Yan Z, Al-Murtadha AA, Kelly DA, McHugh T, Imhof A, Rappsilber J, Jeyaprakash AA,. 2024. PLK1-mediated phosphorylation cascade activates Mis18 complex to ensure centromere inheritance. Science, 6;385(6713):1098-1104

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