Paraffin Sections
- Rehydrate
- 2 x xylene or histoclear
- 2 x absolute alcohol
- 2 x 70% alcohol
- Ehrlichs haematoxylin (5 mins)
- Rinse in tap water
- Differentiate in acid alcohol (15 sec)
- Blue in tap water (5 mins)
- Aqueous eosin (30ml eosin, 300ml water) for 15 mins
- Rinse in tap water
- Dehydrate
- 2 x 70% alcohol
- 2 x absolute alcohol
- 2 x xylene or histoclear
- Mount in DPX
Frozen Sections
- Rehydrate slides in tap water (1 min)
- Full strength Ehrlichs haematoxylin (40 secs)
- Gently rinse in tap water
- Dip 3 times in acid alcohol
- Gently rinse in tap water (5 mins)
- Aqueous eosin (30ml eosin, 300ml water) for 15 mins
- Rinse gently in tap water (5 mins)
- Dehydrate
- 2 x 70% alcohol
- 2 x absolute alcohol
- 2 x xylene or histoclear
- Mount in DPX