Nikon Ti2 CSU-W1 Spinning Disk

Information about Nikon Ti2 CSU-W1 Spinning Disk equipment.

Available Lasers

LaserPower at x20 Objective
405nm2.5mW
488nm6.0mW
561nm7.15mW
633nm9.0mW

Available Filter Sets

FluorophoreExcitationDichroicEmission
DAPI405nmQuad or Triple447/60nm
eGFP488nmQuad or Triple525/50nm or 525/30nm
Cy3561nmQuad or Triple600/52nm or 607/70
Texas Red561nm (~50% efficient)Quad or Triple630/92nm or 647LP
Cy5633nmQuad708/75nm or 647LP
Photo of Nikon Ti2 CSU-W1 Spinning Disk

Software Modules

  • Nikon Elements 5.11 (Also available offline)
  • Nikon Jobs

 

Objectives

TypeMagnificationCorrectionNAContrast Method
Plan Apo VCx100Oil1.4 
Plan Apo Lambdax60Oil1.4 
Plan Apo VCx60Water1.2DIC
Plan Apo VCx20Air0.8DIC
Plan Apo Lambdax10Air0.5 

Supporting Equipment

  • Cherry Temperature Controller 5-45ºC in 10sec shifts
  • Bruker FRAP module
  • Slide Holder
  • 35mm Petri Dish Holder
  • Multiwell Holder

Room Location

Swann 2.16

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