Haematoxylin and Eosin

Haematoxylin and Eosin protocols.

Paraffin Sections

  • Rehydrate
    • 2 x xylene or histoclear

    • 2 x absolute alcohol

    • 2 x 70% alcohol

  • Ehrlichs haematoxylin (5 mins)

  • Rinse in tap water

  • Differentiate in acid alcohol (15 sec)

  • Blue in tap water (5 mins)

  • Aqueous eosin (30ml eosin, 300ml water) for 15 mins

  • Rinse in tap water

  • Dehydrate

    • 2 x 70% alcohol

    • 2 x absolute alcohol

    • 2 x xylene or histoclear

  • Mount in DPX

Frozen Sections

  • Rehydrate slides in tap water (1 min)

  • Full strength Ehrlichs haematoxylin (40 secs)

  • Gently rinse in tap water

  • Dip 3 times in acid alcohol

  • Gently rinse in tap water (5 mins)

  • Aqueous eosin (30ml eosin, 300ml water) for 15 mins

  • Rinse gently in tap water (5 mins)

  • Dehydrate

    • 2 x 70% alcohol

    • 2 x absolute alcohol

    • 2 x xylene or histoclear

  • Mount in DPX

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