Haematoxylin and Eosin

Haematoxylin and Eosin protocols.

Paraffin Sections

  • Rehydrate
    • 2 x xylene or histoclear
    • 2 x absolute alcohol
    • 2 x 70% alcohol
  • Ehrlichs haematoxylin (5 mins)
  • Rinse in tap water
  • Differentiate in acid alcohol (15 sec)
  • Blue in tap water (5 mins)
  • Aqueous eosin (30ml eosin, 300ml water) for 15 mins
  • Rinse in tap water
  • Dehydrate
    • 2 x 70% alcohol
    • 2 x absolute alcohol
    • 2 x xylene or histoclear
  • Mount in DPX

Frozen Sections

  • Rehydrate slides in tap water (1 min)
  • Full strength Ehrlichs haematoxylin (40 secs)
  • Gently rinse in tap water
  • Dip 3 times in acid alcohol
  • Gently rinse in tap water (5 mins)
  • Aqueous eosin (30ml eosin, 300ml water) for 15 mins
  • Rinse gently in tap water (5 mins)
  • Dehydrate
    • 2 x 70% alcohol
    • 2 x absolute alcohol
    • 2 x xylene or histoclear
  • Mount in DPX

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