Spinning Disk Confocal

Information about Spinning Disk Confocal equipment.

Available Lasers

LaserPower at x20 Objective
488nm1.45mW
514nm1.01mW
561nm1.58mW
594nm1.45mW

Available Filter Sets

FluorophoreExcitationDichroicEmissionFilter Set Choices
eGFP488

488/561

488/594

525/50

Dichroic: Chroma zt488/561tpc

Dichroic: Chroma zt488/594tpc

Emission: Chroma ET525/50m

YFP514514/594545/40

Dichroic Semrock Di01-T445/514/593

Emission: Chroma: ET545/40m

tdTomato564488/561590LP

Dichroic: Chroma zt488/561tpc

Emission: Chroma T590lpxr-UF2

mCherry594

514/594

488/594

645/75

Dichroic Semrock Di01-T445/514/593

Dichroic Chroma zt488/594tpc

Emission: Chroma ET645/75m

Photo of Spinning Disk Confocal

Software

  • Metamorph

Available Modules

  • 4D Acquistion
  • ASI Crisp autofocus system for long term timelapse
  • ASI piezo XYZ stage for high accuracy/repeatability
  • Andor iXon EMCCD camera
  • Yokogawa spinning disk unit
  • iLAS FRAP module (GFP only)

Objectives

TypeMagnificationCorrectionNA
Plan Apox20Air, DIC0.75
Plan Fluorx40Air0.75
Plan Apo TIRFx100Oil, DIC1.45

Supporting Equipment

  • Environmental Chamber
    • Temperature control range from ambient to 45°C
    • Humidified CO2 from 1% to 11% available on request
  • Optisplit
    • Simultaneous acquisition of upto 3 fluorophores.
    • Splits camera chip into 2 or 3 regions.

Room Location

March G.4

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