Regulation of the Key Chromosome Components: Condensin, Cohesin and TOP2a Abstract: During mitosis, cells must faithfully segregate meters of entangled DNA into two daughter cells. To do this, DNA is untangled, packaged into chromosomes, and the copied chromosomes are held together at the centromere until the cell is ready to divide. Errors in this process cause genome instability and are associated with human disease, such as developmental disorders and cancer. Three protein complexes; condensin, cohesin and Topoisomerase 2a are essential to this process. Condensin spools DNA into loops to form chromosomes, Topoisomerase 2a untangles the DNA, and cohesin holds the copied chromosomes together. My work has investigated the regulation and mechanism of these crucial proteins. By employing a combination of biochemistry and single molecule approaches I have examined how condensin complexes are activated at the start of mitosis and visualised how cohesin prevents Topoisomerase 2a from untangling DNA.Host: Davide Michieletto Feb 27 2025 09.30 - 10.30 Regulation of the Key Chromosome Components: Condensin, Cohesin and TOP2a Erin Cutts (School of BioSciences, University of Sheffield) Seminar room 1.08, C.H. Waddington Building, Max born Crescent, Kings Buildings Campus Cutts Lab
Regulation of the Key Chromosome Components: Condensin, Cohesin and TOP2a Abstract: During mitosis, cells must faithfully segregate meters of entangled DNA into two daughter cells. To do this, DNA is untangled, packaged into chromosomes, and the copied chromosomes are held together at the centromere until the cell is ready to divide. Errors in this process cause genome instability and are associated with human disease, such as developmental disorders and cancer. Three protein complexes; condensin, cohesin and Topoisomerase 2a are essential to this process. Condensin spools DNA into loops to form chromosomes, Topoisomerase 2a untangles the DNA, and cohesin holds the copied chromosomes together. My work has investigated the regulation and mechanism of these crucial proteins. By employing a combination of biochemistry and single molecule approaches I have examined how condensin complexes are activated at the start of mitosis and visualised how cohesin prevents Topoisomerase 2a from untangling DNA.Host: Davide Michieletto Feb 27 2025 09.30 - 10.30 Regulation of the Key Chromosome Components: Condensin, Cohesin and TOP2a Erin Cutts (School of BioSciences, University of Sheffield) Seminar room 1.08, C.H. Waddington Building, Max born Crescent, Kings Buildings Campus Cutts Lab
Feb 27 2025 09.30 - 10.30 Regulation of the Key Chromosome Components: Condensin, Cohesin and TOP2a Erin Cutts (School of BioSciences, University of Sheffield)
