Publications

Publications from the lab.

 2024 

 

  1. Dharmadhikari, AV ,  Abad MA , Khan S , Maroofian R , Sands TT , Ullah F , Samejima I , Wear MA , Moore KE , Kondakova E , Mitina N , Schaub T , Lee GK , Umandap CH , Berger SM , Iglesias AD , Popp B , Jamra RA , Gabriel H , Rentas S , Rippert AL , Izumi K , Conlin LK , Koboldt DC , Mosher TM , Hickey SE , Albert DV , Norwood H , Lewanda AF , Dai H , Liu P , Mitani T , Marafi D , Pehlivan D , Posey JE , Lippa N , Vena N , Heinzen EL , Goldstein DB , Mignot C , de Sainte Agathe J , Al-Sannaa NA , Zamani M , Sadeghian S , Azizimalamiri R , Seifia T , Zaki MS , Abdel-Salam GM , Abdel-Hamid M , Alabdi L , Alkuraya FS , Dawoud H , Lofty A , Bauer P , Zifarelli G , Afzal E , Zafar F , Efthymiou S , Gossett D , Towne MC , Yeneabat R , Wontakal SN , Aggarwal VS , Rosenfeld JA , Tarabykin V , Ohta S , Lupski JR , Houlden H , Earnshaw WC , Davis EE§ , Jeyaprakash AA§ , Liao J§. RNA Methyltransferase SPOUT1/CENP-32 Links Mitotic Spindle Organisation with the Neurodevelopmental Disorder SpADMiSS. medRxiv (2024) Doi: https://doi.org/10.1101/2024.01.09.23300329  

 

  1. Kruse, T., Garvanska, D. H., Varga, J., Garland, W., McEwan, B., Hein, J. B., Weisser, M. B., Puy, I. B., Chan, C. B., Sotelo-Parilla, P., Mendez, B. L., Jeyaprakash, A. A., Schueler-Fruman, O., Jensen, T. H., Kettenbach, A and Nilsson, J. Substrate Recognition Principles for the PP2A-B55 Protein Phosphatase. Science Advances (2024) 10 (40):eadp5491 Doi: https://doi.org/10.1126/sciadv.adp5491 PMC11446282 

 

  1. Parashara, P*., Medina-Pritchard, B*., Abad, M. A*., Sotelo-Parrilla, P*., Thamkachy, R., Grundei, D., Zou, J., Spanos, C., Kumar, C. N., Basquin, C., Das, V., Yan, Z., Al-Murtadha, A. A., Kelly, D. A., McHug, T., Imhof, A., Rappsilber, J and Jeyaprakash, A. A§. PLK1-Mediated Phosphorylation Cascade Activates Mis18 Complex to Ensure Centromere Inheritance. Science (2024) 285 (6713): 1098-1104. Doi: https://doi.org/10.1126/science.ado8270   

 

  1. Thamkachy, R*., Medina-Pritchard, B*., Park, S. O*., Chiodi, C. G., Zou, J., de la Torre-Barranco, M., Shimanaka, K., Abad, M. A., Páramo, G., Feederle, R., Ruksenaite, E., Heun, P., Davies, O. R., Rappsilber, J., Schneidman-Duhovny, D., Cho., U-S and Jeyaprakash, A. A§. Structural Basis for Mis18 Complex Assembly and its Implications for Centromere Maintenance. EMBO Rep (2024) 25(8):3348-3372  Doi: https://doi.org/10.1038/s44319-024-00183-w  

 

  1. Aktar, K., Davie, T., Leontiou, I., Clark, I., Spanos, C., Wallace, E., Tuck, L., Jeyaprakash, A. A., Hardwick, K. G. Conserved Signalling functions for Mps1, Mad1 and Mad2 in the Cryptococcus neoformans spindle checkpoint. PLoS Genet (2024) 20(6):e1011302 DoI: https://doi.org/10.1371/journal.pgen.1011302.eCollection PMC11175454 

 

  1. Samejima, K., Gibcus., J. H., Abraham, S., Cisneros-Soberanis, F., Samejima, I., Beckett, A. J., Pučeková, N., Abad, M. A., Medina-Pritchard, B., Paulson, J. R., Xie, L., Jeyaprakash, A. A., Prior, I. A., Mirny, L. A., Dekker, J., Goloborodko, A and Earnshaw, W. C. Rules of Engagement for Condensins and Cohesins Guide Mitotic Chromosome Formation. bioRxiv (2024) Doi: https://doi.org/10.1101/2024.04.18.590027 PMC11042376 

 

  1. Manso-Bazus, C., Spataro, N., Gabau, E., brunet-Vega, A., Baena, N., Jeyaprakash, A. A and Ruiz, A. Identification of a Novel Variant p.Gly215Arg in the CHN1 Gene Causing Moebius Syndrome. Front. in. Genetics, 15 (2024). https://doi.org/10.3389/fgene.2024.1291063  

 

 

2023 

 

  1. Rizzotto, A., Tollis, S., Pham, N. T., Zheng, Y., Abad, M. A., Wildenhain, J., Jeyaprakash, A. A., Auer, M., Tyres, M and Schirmer, E. C. Reduction in Nuclear Size by DHRS7 in Prostate Cancer Cells and by Estradiol Propionate in DHRS7-Depeted Cells. Cells (2023) 13(1):57 Doi: https://doi.org/10.3390/cells13010057 PMC10778050 

 

  1. Sankaranarayanan, S. R., Polisetty, S., Dumbrepatil, A., Jeyaprakash, A. A and Sanyal, K. Functional Plasticity in Chromosome-Microtubule Coupling on the Evolutionary Time Scale. Life Science Alliance (2023). Doi: https://doi.org/10.26508/lsa.202201720   

 

  1. London, N., Medina-Pritchard, B., Spanos, C., Rappsilber, J., Jeyaprakash, A. A and Allshire, R. Direct Recruitment of Mis18 to Interphase Spindle Poles Promotes CENP-A Chromatin Assembly. Current Biology,33 (19), 4187-4201. (2023) DoI: https://doi.org/10.1016/j.cub.2023.08.063  

 

  1. Kochanova, N. Y., Abad, M. A., Vizjak, P., Jeyaprakash, A. A., Earnshaw, W. C and Kustatscher, G. ChromatoShiny: an Interactive R/Shiny App for Plotting Chromatography Profiles. Wellcome Open Research (2023) https://wellcomeopenresearch.org/articles/8-332  

 

  1. Vivek, H, S, G., Sotelo-Parrilla, P., Raju, S., Jha, S., Gireesh, A., Gut, F., Vinothkumar, K. R., Berger, F. Jeyaprakash, A. A and Shivaprasad, P. V. Oryza genera-specific Novel Histone H4 Variant Predisposes H4 Lysine5 Acetylation Marks to Modulate Salt Stress Responses. bioRxiv (2023) https://www.biorxiv.org/content/10.1101/2023.07.31.551207v1  

 

2022 

 

  1. Staneva, D. P., Bresson, S., Auchynnikava, T., Spanos, C., Rappsilber, J., Jeyaprakash, A. A., Tollervey, D., Matthews, K. R and Allshire, R. C. The SPARC Complex Defines RNAPII Promoters in Trypanosoma brucei. Elife (2022) https://doi.org/10.7554/eLife.83135. PMC9566855 

 

  1. Leontinou, I., Davies, T., Clark, I., Aktar, K., Suresh, A. P., Abad, M. A., Spanos, C., Lee, K-T., Bahn, Y-S., Jeyaprakash, A. A and Hardwick, K. G. Bub1 Kinase Acts as a Signalling Hub for the Entire Cryptococcus Neoformans Spindle Assembly Checkpoint Pathway. bioRxiv https://www.biorxiv.org/content/10.1101/2022.09.21.508923v1  

 

  1. Kucharski, T. J., Hards, R., Vandal, S. E., Abad, M. A., Jeyaprakash, A. A., Kaye, E., Al-Rawi, A., Ly, T., Godek, K. M., Garber, S. A and Compton, D. A. Small Changes in Phospho-Occupancy at the Kinetochore-Microtubule Interface Drive Mitotic Fidelity. J. Cell Biol (2022) 221(9):e20210710 

 

  1. Abad, M. A*., Gupta, T*., Hadders, M, A., Meppelink, A., Wopken, J. P., Blackburn, E., Zou, J., Gireesh, A., Buzuk, L., Kelly, D, A., McHugh, T., Rappsilber, J., Lens, S. M. A and Jeyaprakash, A. A§. Mechanistic Basis for Sgo1-Mediated Centromere Localisation and Function of the CPC. J. Cell Biol  (2022)  221(8):e202108156 Doi: https://doi.org/10.1083/jcb.202108156  

 

  1. Vit, G., Duro, J., Rajendraprasad, G., Hertz, E. P. T., Katrine, L., Holland, K., Weisser, M. B., McEwan, B. C., Lopez-Mendez, B., Sotelo-Parilla, P., Jeyaprakash, A. A., Montoy, G., Mailand, N., Maeda, K., Kettenbach, A., Barisic, M and Nilsson, J. Chemogenic Profiling Reveals PP2A-independent Cytotoxicity of Proposed PP2A Activators iHAP1 and DT-061. EMBO J (2022) 41(14):e110611. 

 

2021 

 

  1. Ambrose, A. J., Pham, N.T, Sivinski, J., Guimarães, L., Mollasalehi, N., Jimenez, P., Abad, M. A., Jeyaprakash, A. A., Shave, S., Costa-Lotufo, L., La Clair, J. J., Auer, M and Chapman (2021) A two-step resin based approach to reveal survivin-selective fluorescent probes. RSC Chemical Biology. DOI:10.1039/d0cb00122h 

 

  1. Staneva, D. P., Carloni, R., Auchynnikava, T., Tong, P., Jeyaprakash, A. A, Rappsilber, J., Matthews, K. R and Allshire, R. C. A systematic analysis of Trypanosoma brucei chromatin factors identifies novel protein interaction networks associated with sites of transcription initiation and termination.  Genome Res (2021) Doi: https://doi.org/10.1101/gr.275368.121  

 

  1. Aguilera, C., Hümmer, S., Masanas, M., Gabau, E., Guitart, M., Jeyaprakash, A. A., Segura, M. R., Santamaria, A and Ruiz, A.  The Novel KIF1A Missesence Variant (R169R) Strongly Reduces Microtubule Stimulated ATPase Activity and Is Associated With NESCAV Syndrome. Frontiers in Neuroscience (2021) 15:618098. Doi: https://doi.org/10.3389/fnins.2021.618098  

 

  1. Ueki, Y., Hadders, M., Weisser, M. B., Nasa, I., Sotelo-Parrilla, P., Cressey, L. E., Gupta, T., Hertz, E. T., Kruse, T., Montoya, G., Jeyaprakash, A. A., Kettenbach, A., Lens, S. M. A and Nilsson, J (2021) A highly conserved binding pocket on PP2A-B56 is required for hSgo1 binding and cohesion protection during mitosis. EMBO Rep (2021) May11;e52295. Doi: 10.15252/embr.202052295.  

 

  1. Su, B. X., Wang, M., Schaffner, C., Nerusheva, O. O., Clift, D., Spanos, C., Wallek, A., Wu, Y., Wu, Y., Rappsilber, J., Jeyaprakash, A. A., Storchova, Z., Hay, R and Marsten, A. L. Sumoylation stabilizes Stabilizes Sister Kinetochore Biorientation to Allow Timely Anaphase. J. Cell Biol (2021) Jul 5; 220(7):e202005130. Doi:10.1083/jcb.202005130. 

 

2020 

 

  1. Pesenti, E., Liskovykh, M., Okazaki, K., Mallozzi, A., Reid, C., Abad, A. A., Jeyaprakash, A. A., Kouprina, N., Larionov, V., Masumoto, H., Earnshaw, W. C. Analysis of Complex DNA Rearrangements During Early Stages of HAC Formation. ACS Synth. Biol. (2020) 9, 12, 3267-3287. 

 

  1. Wimbish, R. T., DeLuca, K. F., Mick, J. E., Himes, J., Sanchez, I. J., Jeyaprakash, A. A and DeLuca, J. G. Coordination of NDC80 and Ska Complexes at the Kinetochore-Microtubule Interface in Human Cells. Mol Biol Cell (2020) DoI: https://doi.org/10.1091/mbc.E20-05-0286   

 

  1. Medina-Pritchard, B., Lazou, V., Zou, J., Byron O., Abad, M. A., Rappsilber, J., Heun, P and Jeyaprakash, A. A§. Structural Basis for Centromere Maintenance by Drosophila CENP-A Chaperone Cal1. EMBO J (2020) Doi: https://doi.org/10.15252/embj.2019103234 

 

2019 

 

  1. Roure, V., Medina-Pritchard, B., Anselm, E., Jeyaprakash. A. A and Heun, P. Epigentic Inheritance of Centromere Identity in a Heterologous System. Cell Rep (2019) 29(2):464-479.  

 

  1. Abad, M. A., Ruppert, J. G., Buzuk, L., Wear, M. A., Zou, J., Webb, K. M., Kelly, D. A., Voigt, P., Rappsilber, J., Earnshaw, W. C and Jeyaprakash, A. A§. Direct Nucleosome Binding of Borealin Secures Chromosome Association and Function of the CPC. J Cell Biol (2019) 218(12):3912-3925. 

 

  1. Capalbo, L., Mela, I., Abad, M. A., Jeyaprakash, A. A., Edwardson, J. M. and Paolo D’Avino, P. Purification of Recomnbinant ESCRT-III Proteins and Their Use in Atomic Force Microscopy and In Vitro Binding and Phosphorylation Assays.  The ESCRT Complexes: Methods and Protocols, Methods in Molecular Biology (2019), Vol 1998. https://doi.org/10.1007/978-1-4939-9492-2_15. 

 

  1. Leontiou, I., London, N., May, K. M., Ma, Y., Grzesiak, L., Medina-Prtichard, B., Jeyaprakash, A. A., Biggins, S and Hardwick, KG (2019) The Bub1-TPR Domain Interacts with Mad3 to Generate a Robust Spindle Checkpoint Arrest. Current Biology 29(14)2407-2414.  

 

 

2018 

 

  1. Anselm, E., Thomae, A. W., Jeyaprakash, A. A and Heun, P. Oligomerisation of Drosophila Nucleophosmin-like protein is required for its centromere localisation. Nucleic Acid Res (2018) 46(21):11274-11286 

 

  1. Cruz, M., Abad, M. A., van Ijcken, W., Galjart, N., Jeyaprakash, A. A., Maiato, H and Ferrás C. (2018) Mitotic progression, arrest, exit or death is determined by centromere integrity and independent of de novo transcription. eLife 7:e36898 DOI: 10.7554/elLife.36898 

 

  1. de la Roche, M., Hamilton, C., Mortensen, R., Jeyaprakash, A. A., Gosh, S and Anand, P. K. Trafficking of cholesterol to the ER is required for NLRP3 inflammasome activation. J Cell Biol (2018) 217(10):3560-3576. 

 

  1. Ruppert, J. G., Samejima, K., Platani, M., Molina, O., Kimura, H., Jeyaprakash, A. A., Ohta, S and Earnshaw, W. C. Heterochromatin Protein 1a Targets the CPC for Activation at Heterochromatin Before Mitotic Entry. EMBO J (2018) 37(6):e97677 

 

  1. Welburn, J. and Jeyaprakash, A. A§. Mechanisms of Mitotic Kinase Regulation: A Structural Perspective.  Front. Cell Div. Biol. (2018) Feb 5;6:6. doi:10.3389/fcell.2018.00006  

 

2017  

 

  1. Spiller, F*., Medina-Pritchard, M*., Abad, M. A*., Wear, M. A., Molina, O., Earnshaw, W. C and Jeyaprakash, A. A§. Molecular Basis for Cdk1 Regulated Timing of Mis18 Complex Assembly and CENP-A Deposition. EMBO Rep (2017) 18:894-905. Doi:10.15252/embr.201643564. 

 

2016 

 

  1. Molina, O., Vargiu, G., Abad, M. A., Zhiteneva, A., Jeyaprakash, A. A., Masumoto, H., Kouprina, N., Larionov, V and Earnshaw, W. C. Epigenetic Engineering Reveals a Balance Between Histone Modifications and Transcription in Kinetochore Maintenance. Nat Commun (2016) Nov 14;7:13334. doi: 10.1038/ncomms13334. 

 

  1. Capalbo, L., Mela, I., Abad, M. A., Jeyaprakash, A. A., Edwardson, J. M and D’Avino, P. P. The ESCRT-III Component CHMP4C is Regulated Via a Relay Mechanism During Abscission. Open Biol (2016) Oct;6(10).pii: 160248. doi: 10.1098/rsob.160248.  

 

  1. Abad, M. A., Zou, J., Medina-Pritchard, B., Nigg, E. A., Rappsilber, J., Santamaria, A. and Jeyaprakash, A. A§. Ska3 Ensures Timely Mitotic Progression by Interacting Directly With Microtubules and Ska1 Microtubule Binding Domain.  Sci Rep (2016) 6, 34042; doi: 10.1038/srep34042 

 

  1. Subramanian, L., Medina-Pritchard, B., Barton, R., Spiller, F., Kulasegaran-Shylini, R., Radaviciute, G, Allshire, R. C and Jeyaprakash A. A§. Centromere Localization and Function of Mis18 Requires ‘Yippee-Like’ Domain-Mediated Oligomerization. EMBO Rep (2016) 17:196-507. 

 

2015 

 

  1. Papini, D., Langemeyer, L., Abad, M. A., Kerr, A., Samejima, I., Eyers, P. A., Jeyaprakash, A. A., Higgins, J. M. G., Barr, F. A. and Earnshaw, W. C.  TD60 Links RalA GTPase Function to the CPC in Mitosis.  Nat Commun (2015) 6:7678. Doi:10.1038/ncomms8678 

 

  1. Platani, M., Trinkle-Mulchay, L., Porter, M., Jeyaprakash, A. A and Earnshaw, W. C. Mio Depletion Links mTOR Regulation to Aurora A and Plk1 Activation at Mitotic Centrosomes. (2015) J Cell Biol. 210 (1): 45-62.  

 

2014 

 

  1. Abad, M. A., Medina, B., Santamaria, A., Zou, J., Plasberg-Hill, C., Madhumalar, A., Jayachandran, U., Redli, P. M., Rappsilber, J., Nigg, E. A. and Jeyaprakash. A. A§. Structural Basis for Microtubule Recognition by the Human Kinetochore Ska Complex. Nat Commun (2014) Jan13; 5:2964. Doi:10.1038/ncomms3964. 

 

2013 

 

  1. Sharma, A., Pohlentz, G., Bobbili, K. B., Jeyaprakash, A. A., Chandran, T., Mormann, M., Swamy, M. J. and Vijayan, M. The Sequence and Structure of Snake Gourd (Trichosanthes Anguina) Seed Lectin, a Three-Chain Nontoxic Homologue of Type II RIP. Acta Cryst (2013) D69, 1493-1503. 

 

2012 

 

  1. Jeyaprakash, A. A., Santamaria, A., Jayachandran, U., Chan, G., Benda., C., Nigg, E. A. and Conti, E.  Structural and Functional Organization of the Ska Complex, a Key Component of the Kinetochore-Microtubule Interface. Mol Cell (2012) 46, 274-286  

 

  1. Chan, Y. W., Jeyaprakash, A. A., Nigg, E. A and Santamaria, A.  Aurora B controls Kinetochore-Microtubule Attachments by Inhibiting Ska Complex-KMN Network Interaction. J Cell Biol (2012) 196, 563-571.  

 

2011 

 

  1. Jeyaprakash, A. A§., Basquin, C., Jayachandran, U. and Conti, E§.  Structural Basis for the Recognition of Phosphorylated Histone H3 by the Survivin Subunit of the Chromosomal Passenger Complex.  Structure (2011) 19, 1625-1634. 

 

2009 

  

  1. Gaitanos, T, N., Santamaria, A*., Jeyaprakash, A. A*., Wang, B., Conti, E. and Nigg, E. A.  Stable Kinetochore-Microtubule Interactions Depend on the Ska Complex and its New Component Ska3/C13orf3) EMBO J (2009) 28, 1442-52. (*Equal Contribution) 

 

2007 

 

  1. Jeyaprakash, A. A*., Klein, U. R*., Lindner, D., Ebert, J., Nigg, E. A. and Conti,  E.  Structure of a Survivin-Borealin-INCENP Core Complex Reveals How Chromosomal Passengers Travel Together. Cell (2007) 131, 271-285.  

 

2005 

 

  1. Jeyaprakash, A. A., Jayashree, G., Mahanta, S. K., Swaminathan, C. P., Sekar, K., Surolia, A. and Vijayan, M.  Structural Basis for the Energetics of Jacalin-Sugar Interactions: Promiscuity Versus Specificity.  J. Mol. Biol. (2005) 347, 181-188. 

 

2004 

 

  1. Jeyaprakash, A. A., Srivastav, A., Surolia, A and Vijayan, M. Structural Basis for the Carbohydrate Specificities of Artocarpin: Variation in the Length of the Loop as a Strategy for Generating Ligand Specificity. J. Mol. Biol. (2004) 338, 757-770. 

 

  1. Natchiar, S. K., Jeyaprakash, A. A., Ramya, T. N. C., Thomas, C. J., Suguna, K., Surolia, A and Vijayan, M. Structural Plasticity of Peanut Lectin: An X-ray Analysis Involving Variation in pH, Ligand Binding and Crystal Structure. Acta Cryst (2004) D60, 211-219. 

 

2003 

 

  1. Sekar, K., Mala, S. V., Rajakannan, V., Yogavel, M., Velmurugan, D., Poi, M-J., Vishwanath, S., Gowda, T. V., Jeyaprakash, A. A. and Tsai, M. D. Crystal Structures of the Free and Anisic Acid Bound Triple Mutant of Phospholipase A2. J. Mol. Biol. (2003) 333, 367-76. 

 

  1. Jeyaprakash, A. A., Katiyar, S., Swaminathan, C. P., Sekar, K., Surolia, A and Vijayan, M.  Structural Basis of the Carbohydrate Specificities of Jacalin: An X-Ray and Modeling Study. J. Mol.  Biol. (2003) 332, 217-28. 

 

2002 

 

  1. Rajakannan, V., Yogavel, M., Poi, M. J., Jeyaprakash, A. A., Jeyakanthan, J., Velmurugan, D., Tsai, M. D and Sekar, K. Observation of Additional Calcium Ion in the Crystal Structure of the Triple Mutant K56,120,121M of Bovine Pancreatic Phospholipase A2. J. Mol. Biol. (2002) 324, 755-62. 

 

  1. Jeyaprakash, A.A., Rani, P. G., Reddy, G. B., Banumathi, S., Betzel, C., Sekar, K., Surolia, A and Vijayan, M. Crystal Structure of the Jacalin-T-antigen Complex and a Comparative Study of Lectin-T-antigen Complexes. J. Mol. Biol. (2002) 321, 637-45. 

 

  1. Pratap, J. V, Jeyaprakash, A. A., Rani, P. G., Sekar, K., Surolia, A and Vijayn, M. Crystal Structures of Artocarpin, a Moraceae Lectin with Mannose Specificity, and its Complex with Methyl-alpha-D-mannose: Implications to the Generation of Carbohydrate Specificity. J. Mol. Biol. (2002) 317, 237-47. 

 

2001 

 

  1. Adhikari, P., Bachhawat-Sikder, K., Thomas, C. J., Ravishankar, R., Jeyaprakash, A. A., Sharma, V., Vijayan, M and Surolia, A. Mutational Analysis at Asn-41 in Peanut Agglutinin. A Residue Critical for the Binding of the Tumor-Associated Thomsen-Friedenreich Antigen. J. Biol. Chem. (2001) 276, 40734-9. 

 

  1. Manoj, N., Jeyaprakash, A. A., Pratap, J. V., Komath, S. S., Kenoth, R., Swamy, M. J. and Vijayan, M. Crystallization and Preliminary X-Ray Studies of Snake Gourd Lectin: Homology with Type II Ribosome-Inactivating Proteins”. Acta Cryst  (2001) D57, 912-4. 

 

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