All links direct to the respective project pages on the OpenWetWare Wiki website (https://openwetware.org/wiki/).
Outline of the protocols written by the Chris French group.
Biobrick Protocols
1. Primer Design.
2. a) Cloning parts by PCR with Pfu polymerase.
2. b) Cloning parts by PCR with Kod polymerase.
3. Agarose Gel Electrophoresis.
4. Purifying a PCR product from solution.
5. Cloning a PCR product into a biobrick vector.
6. Preparing and using competent E. coli cells.
8. a) Plasmid DNA minipreps.
8. b) Plasmid DNA maxipreps.
9. Analytical restriction digests.
10. Combining two biobricks to make a new biobrick.
11. Adding a ribosome binding site to a biobrick coding sequence.
12. BALTIC: a new method for combining standard BioBricks.
13. MABEL: a fast easy method for removing unwanted restriction sites.
14. BABEL: a new method for generating BioBricks without restriction digests.
Other Protocols
1. Growth of E. coli.
2. Growth of B. subtilis.
3. a) Transforming Bacillus subtilis.
3. b) Transforming Bacillus subtilis – 2.
5. Restriction digests.
6. Agarose gel electrophoresis.
7. PCR.
8. Purification of DNA fragments.
9. Ligation.
10. Cloning of PCR products.
11. Preparation of cell extracts.
12. Protein Assay.
13. SDS-PAGE.
14. Native-PAGE.
15. Enrichment and growth of environmental bacteria.
16. Identification of bacteria.
17. Gram stain.
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