Please email me for reprints/pdfs. You can also see my publications on Google Scholar or follow me on Research Gate.
Publications from the lab, including open access links.
2024
Twyford, A.D. et al., 2024. A DNA barcoding framework for taxonomic verification in the Darwin Tree of Life Project. Wellcome Open Research, 9, p.339. [Open access]
Brown, M. R., Abbott, R. J., Twyford, A.D. (2024) The emerging importance of cross‐ploidy hybridisation and introgression. Molecular Ecology, e17315. [Open access]
Christenhusz, M. J. M., Pannell, J., R., Twyford, A. D., (2024) Wellcome Sanger Institute Tree of Life, and Darwin Tree of Life Consortium. (2024) The genome sequence of the Annual Mercury, Mercurialis annua L., 1753 (Euphorbiaceae). Wellcome Open Research 9. [Open access]
2023
Brown, M. R., Hollingsworth, P. M., Forrest, L. L., Hart, M. L., Leitch, I. J., Jones, L. J., Ford, C., De Vere, N., Twyford, A. D. (2023) Genetic factors predict hybrid formation in the British flora. Proceedings of the National Academy of Sciences 120 (16): e2220261120. [Open access]
Boyle, J.H., Strickler, S., Twyford, A.D., Ricono, A., Powell, A., Zhang, J., Xu, H., Smith, R., Dalgleish, H.J., Jander, G., Agrawal, A.A., Puzey, J. (2023) Temporal matches between monarch butterfly and milkweed population changes over the past 25,000 years. Current Biology, 33(17), pp.3702-3710. [Open access]
Brown, M. R., Becher, H., Williams, S., Twyford, A. D. (2023) Is there hybridization between diploid and tetraploid Euphrasia in a secondary contact zone? American Journal of Botany 110, no. 1 (2023): e16100. [Open access]
Barnbrook, M., Durán‐Castillo, M., Critchley, J., Wilson, Y., Twyford, A. D., Hudson, A. (2023). Recent parallel speciation in Antirrhinum involved complex haplotypes and multiple adaptive characters. Molecular Ecology, 32(19), 5305-5322. [Open access]
Fu, P.C., Twyford, A.D., Hao, Y.T., Zhang, Y., Chen, S.L., Sun, S.S. (2023) Hybridization and divergent climatic preferences drive divergence of two allopatric Gentiana species on the Qinghai–Tibet Plateau. Annals of Botany, 132(7), pp.1271-1288. [Open access]
Christenhusz, M. J., Twyford, A. D., Hudson, A., & Darwin Tree of Life Consortium. (2023). The genome sequence of thale cress, Arabidopsis thaliana (Heynh., 1842). Wellcome Open Research, 8(40), 40. [Open access]
Christenhusz, M. J., Bell, D., Twyford, A. D., & Darwin Tree of Life Consortium. (2023). The genome sequence of common ivy, Hedera helix L., 1753. Wellcome Open Research, 8, 325. [Open access]
2022
Durán-Castillo, M., Hudson, A., Wilson, Y., Field, D. L., Twyford, A. D. (2022) A phylogeny of Antirrhinum reveals parallel evolution of alpine morphology. New Phytologist 233 (3): 1426-1439. [Open access]
Soza, V.L., Kriebel, R., Ramage, E., Hall, B.D. and Twyford, A.D. (2022) The symmetry spectrum in a hybridising, tropical group of rhododendrons. New Phytologist, 234: 1491-1506. [Open access]
Darwin Tree of Life Project Consortium (2022) Sequence locally, think globally: The Darwin Tree of Life Project. Proceedings of the National Academy of Sciences 119 (4), e2115642118. [Open access]
Garrett, P., Becher, H., Gussarova, G., dePamphilis, C.W., Ness, R.W., Gopalakrishnan, S., Twyford, A.D. (2022) Pervasive phylogenomic incongruence underlies evolutionary relationships in eyebrights (Euphrasia, Orobanchaceae). Frontiers in Plant Science. 13:869583. [Open access]
Becher H., Sampson, J., Twyford, A.D. (2022) Measuring the invisible: the sequences causal of genome size differences in eyebrights (Euphrasia) revealed by k-mers. Frontiers in plant science: 2410. doi.org/10.3389/fpls.2022.818410.
Qi, S., Twyford, A. D., Ding, J., Borrell, J. S., Ma, Y., Wang, N., (2022) Natural interploidy hybridization among the key taxa involved in the origin of horticultural chrysanthemums. Journal of Systematics and Evolution. https://doi.org/10.1111/jse.12810.
Schley, R. J., Twyford, A. D., Pennington R.T. (2022) Hybridization: a ‘double-edged sword’ for Neotropical plant diversity. Botanical Journal of the Linnean Society 199 (1), 331-356.
Nishii, K., Hart, M., Kelso, N., Barber, S., Chen, Y.-Y., Thomson, M., Trivedi, U., Twyford, A. D., Möller, M. (2022). The first genome for the Cape Primrose Streptocarpus rexii (Gesneriaceae), a model plant for studying meristem-driven shoot diversity. Plant Direct 6 (4), e388.
Henniges, M., Powell, R., Mian, S., Stace, C., Walker, K., Gornall, R., Christenhusz, M., Brown, M., Twyford, A. D., Hollingsworth, P. M., Jones, L., De Vere, N., Antonelli, A., Leitch, A., Leitch I. (2022) A taxonomic, genetic and ecological data resource for the vascular plants of Britain and Ireland. Scientific Data 9 (1), 1-8.
Liu, Z. F., Ma, H., Zhang, X. Y., Ci, X. Q., Li, L., Hu, J. L., Zhang, C. Y., Xiao, J. H., Li, H. W., Conran, J.G. and Twyford, A.D., Hollingsworth, P. M., Li, J. (2022) Do taxon-specific DNA barcodes improve species discrimination relative to universal barcodes in Lauraceae? Botanical Journal of the Linnean Society boab089, https://doi.org/10.1093/botlinnean/boab089.
2021
Brown, M., Frachon, N., Moore, P.M., Twyford, A.D. (2021) Performance of generalist hemiparasitic Euphrasia across a phylogenetically diverse host spectrum. New Phytologist 232 (5): 2165–2174.
Becher, H., Powell, R. F., Brown, M. R., Metherell, C., Pellicer, J., Leitch, I. J., Twyford, A. D. (2021) The nature of intraspecific genome size variation in taxonomically complex eyebrights. Annals of Botany 128 (5): 639–651
Ranavat, S., Becher, H., Newman, M. F., Gowda, V., Twyford, A. D. (2021) A draft genome of the ginger species Alpinia nigra and new insights into the genetic basis of flexistyly. Genes 12 (9) 1297.
Jones, L., Twyford, A.D., Ford, C. R., Rich, T. C. G., Davies, H., Forrest, L. L., Hart, M. L., McHaffie, H., Brown, M. R., Hollingsworth, P. M., de Vere, N. (2021) Barcode UK: A complete DNA barcoding resource for the flowering plants and conifers of the United Kingdom. Molecular Ecology Resources 21 (6), 2050-2062.
Fu, P. C., Twyford, A. D., Sun, S. S., Wang, H. Y., Xia, M. Z., Tan, C. X., Zhou, X. J., Chen, S. L., (2021) Recurrent hybridization underlies the evolution of novelty in Gentiana (Gentianaceae) in the Qinghai-Tibetan Plateau, AoB PLANTS, 13 (1), plaa068.
Fu, P. C., Sun, S. S., Twyford, A. D., Li, B. B., Zhou, R. Q., Chen, S. L., Gao, Q. B., Favre, A., (2021) Lineage‐specific plastid degradation in subtribe Gentianinae (Gentianaceae). Ecology and Evolution 00: 1– 14.
Vallejo-Marín, M., Friedman, J., Twyford, A. D., Lepais, O., Ickert-Bond, S. M., Streisfeld, M. A., Yant, L., van Kleunen, M., Rotter, M. C., Puzey, J. R. (2021) Population genomic and historical analysis reveals a global invasion by bridgehead processes in Mimulus guttatus. Communications Biology 4 (1): 1-12.
Liu et al. (2021), Can plastid genome sequencing be used for species identification in the Lauraceae? Botanical Journal of the Linnean Society 197 (1) 1–14.
2020
Twyford, A. D., Wong, E. L. Y., Friedman (2020) Multi-level patterns of genetic structure and isolation by distance in the widespread plant Mimulus guttatus. Heredity 125 (4), 227-239.
Becher, H., Brown, M. R., Powell, G., Metherell, C., Riddiford, N. J., Twyford, A. D. (2020) Maintenance of species differences in closely related tetraploid parasitic Euphrasia (Orobanchaceae) on an isolated island. bioRxiv: https://doi.org/10.1101/2020.04.29.067579
Clubbe, C. A. et al. (2020) Current knowledge, status and future for plant and fungal diversity in Great Britain and the UK Overseas Territories. Plants, People, Planet (In Press).
Twyford A. D., Frachon, N., Wong, E. L. Y., Metherell, C., Brown, M. R. (2020) Life history evolution and phenotypic plasticity in parasitic eyebrights (Euphrasia, Orobanchaceae). American Journal of Botany 107 (3), 456-465 [PDF]
2018
Twyford A.D. (2018) Parasitic Plants. Current Biology 28 (16), R857-R859. [PDF]
Twyford A.D. (2018) The road to 10,000 plant genomes. Nature Plants 4 (6), 312-313. [Open access]
Wang X, Gussarova G, Ruhsam M, de Vere N, Metherell C, Hollingsworth PM, Twyford AD. (2018) DNA barcoding a taxonomically complex hemiparasitic genus reveals deep divergence between ploidy levels but lack of species-level resolution. AoB PLANTS, 10: ply026. [Open Access]
Twyford, A. D., Caola, A. M., Choudhary, P., Raina, R., Friedman, J. (2018) Loss of color pigmentation Is maintained at high frequency in a monkey flower population. The American Naturalist. 191 (1). [Open Access]
2017
Quinteros Peñafiel, C. V., Riddiford, N. J., Twyford, A. D. (2017) A floritistic survey of Fair Isle. New Journal of Botany. 2-3:101-111. [Open Access]
Twyford, A. D. (2017) New insights into the population biology of endoparasitic Rafflesiaceae. American Journal of Botany 104 (10), 1433-1436 [link, Open Access]
Dexter K. G., Lavin, M., Torke, B. M., Twyford, A. D., Kursar, T. A., Coley, P. D., Drake, C., Hollands, R., Pennington R. T. (2017) Dispersal assembly of rain forest tree communities across the Amazon basin. Proceedings of the National Academy of Sciences. 114, 2645-2650. [Open access] [BBC News coverage]
2016
Twyford, A. D., Ness, R. W. (2016) Strategies for complete plastid genome sequencing. Molecular Ecology Resources. [Open Access]
Hollingsworth, P. M., De-Zhu, L., Van der Bank, M., Twyford, A. D. (2016) Telling plant species apart with DNA: from barcodes to genomes. Philosophical Transactions of the Royal Society B. 371 (1702), 20150338 [Open Access]
Twyford, A. D. (2016) Will benchtop sequencers resolve the sequencing trade-off in plant genetics? Frontiers in Plant Science 7: 433. [Open Access]
2015
Twyford, A. D., Kidner, C. A., Ennos, R. A. (2015) Maintenance of species boundaries in a Neotropical radiation of Begonia. Molecular Ecology 24 (19), 4982-4993. [Open Access]
Twyford, A. D., Friedman, J. (2015) Adaptive divergence in the monkey flower Mimulus guttatus is maintained by a chromosomal inversion. Evolution 69 (6), 1476-1486. [Open Access]
Twyford, A. D., Streisfeld, M. A., Lowry, D. B., Friedman, F. (2015) Genomic studies on the nature of species: adaptation and speciation in Mimulus. Molecular Ecology. In Press [Open Access]
Friedman, J., Twyford, A. D., Willis, J. H., Blackman, B. K. (2015) The extent and genetic basis of phenotypic divergence in life history traits in Mimulus guttatus. Molecular ecology 24(1), 111-122 [Open access]
Chan,Y. M., Twyford, A. D., Tnaha, L. H., Lee, C. T. (2015) Characterisation of EST-SSR markers for Begonia maxwelliana (Begoniaceae) and cross-amplification in 23 species from 7 Asian sections. Scientia Horticulturae 190 (2015) 70–74.
2014
Twyford, A. D. (2014) Testing evolutionary hypotheses for DNA barcoding failure in willows. Molecular Ecology 23(19):4674-6. [Open access]
Twyford, A. D., Kidner, C. A., Ennos, R. A. (2014) Genetic differentiation and species cohesion in two widespread Central American Begonia (Begoniaceae) species. Heredity 112, 382-390. [Open access]
Twyford, A.D., Ennos, R.A., White, C.D., Shaukat Ali, M., Kidner, C.A. (2014) The evolution of sex ratio differences and inflorescence architectures in Begonia (Begoniaceae). American Journal of Botany 101(2):308-317. [Open access]
Cruz Neto, O., Aguiar, A.V., Twyford, A. D., Neaves, L.E., Pennington, R.T., Lopes, A.V. (2014) Genetic and ecological outcomes of Inga vera subsp. affinis (Leguminosae) tree plantations in a fragmented tropical landscape. PLOS One 9(6): e99903. [Open access]
2013
Twyford, A. D., Kidner, C. A., Harrison, N., Ennos, R. A. (2013) Population history and seed dispersal in widespread Central American Begonia (Begoniaceae) species inferred from plastome derived microsatellite markers. Botanical Journal of the Linnean Society. 171: 260-276. [Open access] Invited article as part of a special issue on Neotropical Evolution.
Twyford, A. D., Ennos, R. A., Kidner, C. A. (2013) Development and characterization of microsatellite markers for Central American Begonia Sect. Gireoudia (Begoniaceae). Applications in Plant Sciences 1(5):1200499. [Open access]
2012
Twyford, A.D., Ennos, R.A., (2012) Next-generation hybridization and introgression. Heredity 108: 179-189. [Open access]
Twyford, A.D., Ennos, R.A. (2012) Next-generation sequencing as a tool for plant ecology and evolution. Plant Ecology and Diversity 5(4):411-413. [Open access]
Brennan, A.C., Bridgett, S., Ali, M.S., Harrison, N., Matthews, A., Pellicer, J., Twyford, A.D., Kidner, C.A. (2012) Genomic resources for evolutionary studies in the large, diverse, tropical genus, Begonia. Tropical Plant Biology 5: 261-276. [Open access]
2011
Thomas, D. C., Hughes, M., Phutthai, T., Ardi, W. H., Rajbhandary, S., Rubite, R., Twyford, A. D., Richardson, J. E. (2011) West to east dispersal and subsequent rapid diversification of the mega-diverse genus Begonia (Begoniaceae) in the Malesian archipelago. Journal of Biogeography 39 (1): 1365-2699. [Open access]
Book Chapters
Hollingsworth, P. M., Neaves, L. E., Twyford, A. D. (2017) Using DNA sequence data to enhance understanding and conservation of plant diversity at the species level. In Plant Conservation Science and Practice: The Role of Botanic Gardens. Blackmore, S. & Oldfield, S. (eds.). Cambridge: Cambridge University Press, p. 23-48.
Twyford, A. D., Richardson, J. E. (2015) Molecular phylogenetics and biogeography of Vireya Rhododendrons. In: Rhododendrons of subgenus Vireya. Argent, G. London: Royal Horticultural Society and the Royal Botanic Garden Edinburgh. 2nd Edition. pp 389-394.
Dewitte, A., Twyford, A.D., Thomas, D.C., Kidner, C.A., Van Huylenbroeck, J., (2011) The origin of diversity in Begonia: genome dynamism, population processes and phylogenetic patterns. In: The Dynamical Processes of Biodiversity - Case Studies of Evolution and Spatial Distribution. Venora, G., Grillo, O., López-Pujol, J., eds Intech Press. Open access.
Other publications
Øvstebø, G., Twyford, A.D., Westerlund, T., (2011) Propagation of dry habitat fern species using spore collections from historic herbarium specimens. Sibbaldia 9: 43-54
Twyford, A.D., Kidner, C.A., (2009) The Evolution of Begonia section Gireoudia. The Begonian 76(6):213-215
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