What we offer

High-performance analysis and sorting capabilities.

Flow Cytometry is a powerful, laser-based technology, which can be used to analyse the physical, chemical and biological properties of individual cells or cellular components, and it permits the analysis of millions of cells in suspension, in a relatively small amount of time.

The facility operates four instruments: Two cell sorters – BD FACS ARIA IIu & BD FACS Discover S8 and three analyzers – BD FACS Canto II, BD Fortessa and Sony ID7000.

Cell Sorters

BD FACS ARIA IIu

The FACSAria IIu high-speed, fixed – alignment cuvette cell sorter can detect up to 14 different parameters +2 FCS/SSC and capable of sorting up to 4 populations at a time.

Flow Core staff operate the FACS AriaIIu sorter.

Temperature control (cooling and heating) is available for collection tubes as well as plates.
Lasers: 4 - 405nm, 488nm, 561nm, 633 nm.
Sample tubes: 5mL FACS tubes, 15mL Falcon tubes.
Collection tubes: FACS tubes, 15mL Falcon tubes, 6/24/48/96/ 384 well plates.

BD FACS Discover S8

The BD FACSDiscover™ S8 Cell sorter is the first cell sorter that combines full-spectrum flow cytometry with sort-capable image analysis. Besides sorting large fluorescent panels, this technology enables sorting based on specific signal localization within the cell or other morphological features.

Flow Core staff operate the BD FACSDiscover™ S8.

Temperature control (cooling and heating) is available for collection tubes as well as plates.
Spectral arrays – 78 fluorescence detectors covering the full spectrum:
- UV 349-nm laser – 22 UV detectors, covering 365–860 nm
- Violet 405-nm laser – 20 Violet detectors, covering 410–860 nm
- Blue 488-nm laser – 16 Blue detectors, covering 495–860 nm
- Y/G 561-nm laser – 12 Yellow-green detectors, covering 570–860 nm
- Red 637-nm laser – 8 Red detectors, covering 645–860nm
Fluorescence detectors for imaging:
- FL1: LP505: 534/46
- FL2: LP570: 600/60
- FL3: LP675: 788/225
Sample tubes: 5mL FACS tubes ONLY
Collection tubes: FACS tubes, 6/24/48/96/ 384 well plates.

Cell Analysers

BD FACS Canto II

The BD FACS Canto II is a benchtop analyser capable of detecting 8 fluorescence channels and 2 channels for Forward Scatter and Side Scatter.

There is the option to run plates with the HTS plate loader for high throughput analysis.

Users undergo training provided by Flow Core staff and are then able to operate the cytometer independently, but staff are always available to address any queries or issues.

Lasers: 5 - 355nm 405nm, 488nm, 561nm, 640nm
Sample tubes: 5mL FACS polystyrene tubes, 96 –well plate using an HTS plate reader.

BD Fortessa

The BD Fortessa is a benchtop analyser capable of detecting up to 16 parameters +2 FSC/SSC.

There is the option to run plates with the HTS plate loader for high throughput analysis.

Users undergo training provided by Flow Core staff and are then able to operate the cytometer independently, but staff are always available to address any queries or issues.

Lasers: 4 lasers - Violet (405), Blue (488), Red (640) and Violet (405)
Sample tubes: 5mL FACS polystyrene tubes, 96 –well plate using an HTS plate reader.

Sony ID7000

The Sony ID7000™ Spectral Cell Analyzer is a cutting-edge cytometer capable of high-parameter spectral analysis, using 7 lasers and 186 detectors to detect rare and dim populations with exceptional sensitivity.

Lasers: 7 - 320 nm, 355 nm, 405 nm, 488 nm, 561 nm, 637 nm, 808 nm
Fluorescence detectors: Photomultiplier tubes (32-channel PMTs, single-channel PMTs, and single-channel IR-sensitive PMTs).
Sample tubes: 5mL FACS polystyrene tubes, 96 –well plate, 384 well-plate using an HTS plate reader.

This article was published on 2024-06-17