The system is built upon the principles of interference reflection and interferometric scattering microscopy and allows for rapid single-particle analysis of biomolecules in solution. It measures the light scattered by individual particles and uses the signal to count the particles and measure their mass. Scattering intensity correlates to mass for a variety of biomolecules, including glycoproteins, nucleic acids and / or lipids), making mass photometry a technique applicable for analysis of all biomolecules in solution. It can also provide the mass distribution and relative concentrations of the biomolecules in a sample.
The range of particles that can be measured/detected by the system lies between 50 kDa. and 5 MDa. Sample volumes are in the 10 - 20 µL range, with a concentration range of ~ 100 nM - 0.1 nM (sample/mass-distribution dependant) for the manual SOP, and 50 µM - 5 nM for the automated MassFluidics (coming on-line soon).
For more information, please click here or contact the EPPF staff.
