Publications from the lab. See full list in Pubmed2026Clifford G and Akiyoshi B (2026) Auxin-inducible degron-mediated protein depletion in Diplonema papillatum. Wellcome Open Research Clifford G, Taylor SJP, Ishii M, Cisneros-Soberanis F, Akiyoshi B (2026) Identification of feeding apparatus components in a heterotrophic marine flagellate. Journal of Cell Science Ishii M and Akiyoshi B (2026) Degrade Green Fluorescent Protein (deGradFP) Method in Trypanosoma brucei. Methods in Molecular Biology 2026;3069:203-212Akiyoshi B (2026) Euglenozoa are paraphyletic if the eukaryotic root lies between glycomonads and the rest of eukaryotes. Journal of Cell Science 139(3):jcs264522. doi: 10.1242/jcs.264522Prokopchuk G, Tashyreva D, Iorillo O, Akiyoshi B, Lukeš J, Faktorová D (2026) Preparation of diplonemid samples for various microscopy techniques. Methods in Molecular Biology 2026;3013:41-56Faktorová D, Valach M, Iorillo O, Akiyoshi B, Burger G, Lukeš J (2026) Genetic manipulation of Paradiplonema papillatum. Methods in Molecular Biology 2026;3014:3-25Ciszek A, Ishii M, Akiyoshi B (2026) Immunoprecipitation of protein complexes from Trypanosoma brucei. Methods in Molecular Biology 2026;3014:113-1212025Giunta S, Fukagawa T, O’Neill RJ, Akiyoshi B (2025) 40 years of CENP-A: the foundation of a new era of centromere biology. Chromosome Res 22;33(1):32 and Chromosoma 134;13Hammond M, Iorillo O, Faktorová D, Svobodová M, Akiyoshi B, Licknack T, Poh Y, Lukeš J, Wideman JG (2025) Subcellular proteomics of model diplonemid Paradiplonema papillatum reveals novel enzymatic compartmentalization and provides new metabolic insights. PLoS Biology 23(12): e3003319Jetishi C, Aeschlimann S, Schimanski B, Käser S, Mullner R, Oeljeklaus S, Akiyoshi B, Warscheid B, Butter F, Schneider A, Ochsenreiter T (2025) Connecting basal body and mitochondrial DNA: TAC53 and the tubular organisation of the tripartite attachment complex. PLoS Pathogens 15;21(9):e1013521 Akiyoshi B, Faktorová D, Lukeš J (2025) Discovery of unique mitotic mechanisms in Paradiplonema papillatum. Open Biology 15(8):250096Akiyoshi B (2025) Hypothesis that ancestral eukaryotes sexually proliferated prior to the evolution of kinetochores or mitosis. Journal of Cell Science 138(11):jcs263843Ludzia P, Nugent C, Akiyoshi B, Redfield C (2025) 1H, 13C and 15N resonance assignments for the acetyltransferase domain of the kinetoplastid kinetochore protein KKT23 from Trypanosoma brucei. Biomolecular NMR Assignments 19(1):187-194Ludzia P, Ishii M, Deák G, Spanos C, Wilson MD, Redfield C, Akiyoshi B (2025) Kinetoplastid kinetochore protein KKT23 acetyltransferase is a structural homolog of GCN5 that acetylates the histone H2A C-terminal tail. Structure 33(1):123-135.e102024Benz C, Raas MWD, Tripathi P, Faktorová D, Tromer EC, Akiyoshi B, Lukeš J (2024) On the possibility of yet a third kinetochore system in the protist phylum Euglenozoa. mBio https://doi.org/10.1128/mbio.02936-24Ballmer D, Lou HJ, Ishii M, Turk BE, Akiyoshi B (2024). An unconventional regulatory circuitry involving Aurora B controls anaphase onset and error-free chromosome segregation in trypanosomes. Journal of Cell Biology https://doi.org/10.1083/jcb.202401169 Ballmer D, Carter W, van Hooff JJE, Tromer EC, Ishii M, Ludzia P, Akiyoshi B# (2024) Kinetoplastid kinetochore proteins KKT14-KKT15 are divergent Bub1/BubR1-Bub3 proteins. Open Biology https://doi.org/10.1098/rsob.240025Ballmer D, Akiyoshi B (2024). Dynamic localization of the chromosomal passenger complex in trypanosomes is controlled by the orphan kinesins KIN-A and KIN-B. elife https://doi.org/10.7554/eLife.93522Ludzia P, Hayashi H, Robinson T, Akiyoshi B, Redfield C (2024). NMR study of the structure and dynamics of the BRCT domain from the kinetochore protein KKT4. Biomolecular NMR Assignment https://doi.org/10.1007/s12104-024-10163-92022Ishii M, Ludzia P, Marcianò G, Allen W, Nerusheva OO, Akiyoshi B (2022) Divergent polo boxes in KKT2 bind KKT1 to initiate the kinetochore assembly cascade in Trypanosoma brucei. Molecular Biology of the Cell (8A0J, 8A0K)López-Escobar L, Hänisch B, Halliday C, Ishii M, Akiyoshi B, Dean S, Sunter JD, Wheeler RJ, Gull K. (2022) Stage-specific transcription activator ESB1 regulates monoallelic antigen expression in Trypanosoma brucei. Nature MicrobiologyIshii M and Akiyoshi B (2022) Targeted protein degradation using deGradFP in Trypanosoma brucei. Wellcome Open ResearchIshii M and Akiyoshi B (2022) Plasticity in centromere organization and kinetochore composition: lessons from diversity. Current Opinion in Cell Biology 2021Ludzia P and Akiyoshi B (2021) Illuminating the mechanism of monogenic antigen expression in trypanosomes. Nature Reviews MicrobiologyMarcianò G, Ishii M, Nerusheva OO, and Akiyoshi B. (2021) Kinetoplastid kinetochore kinases KKT2 and KKT3 have unique centromere localization. Journal of Cell Biology (6TLX, 6LTY)Tromer EC, Wemyss TA, Ludzia P, Waller RF, and Akiyoshi B (2021) Repurposing of synaptonemal complex proteins for kinetochores in Kinetoplastida. Open Biology 11: 210049Ludzia P, Lowe ED, Marcianò G, Mohammed S, Redfield C, and Akiyoshi B (2021) Structural characterisation of KKT4, an unconventional microtubule-binding kinetochore protein. Structure doi.org/10.1016/j.str.2021.04.004 (6ZPM, 6ZPJ, 6ZPK)2020Akiyoshi B (2020, Preprint) Analysis of a Mad2 homolog in Trypanosoma brucei provides possible hints on the origin of the spindle checkpoint. bioRxivLudzia P, Akiyoshi B, and Redfield C (2020). 1H, 13C and 15N resonance assignments for the microtubule-binding domain of the kinetoplastid kinetochore protein KKT4 from Trypanosoma brucei. Biomolecular NMR Assignments 14: 309–315Ishii M and Akiyoshi B (2020) Characterization of unconventional kinetochore kinases KKT10 and KKT19 in Trypanosoma brucei. Journal of Cell Science 133(8): jcs2409782019Nerusheva OO, Ludzia P, and Akiyoshi B. (2019) Identification of four unconventional kinetoplastid kinetochore proteins KKT22–25 in Trypanosoma brucei. Open Biology 9: 190236Akiyoshi B. (2019) Evolution: A Mosaic-type Centromere in an Early-Diverging Fungus. Current Biology 29: R1184-R11862018Llauró A, Hayashi H, Bailey ME, Wilson A, Ludzia P, Asbury CL, and Akiyoshi B. (2018) The kinetoplastid kinetochore protein KKT4 is an unconventional microtubule tip-coupling protein. Journal of Cell Biology 217(11): 3886-3900Hayashi H and Akiyoshi B. (2018). Degradation of cyclin B is critical for nuclear division in Trypanosoma brucei. Biology Open 23;7(3). pii: bio031609 2017Drinnenberg IA and Akiyoshi B (2017) Evolutionary Lessons from Species with Unique Kinetochores. In: Black B. (eds) Centromeres and Kinetochores. Progress in Molecular and Subcellular Biology, vol 56. Springer, Cham2016Nerusheva OO and Akiyoshi B. (2016) Divergent polo box domains underpin the unique kinetoplastid kinetochore. Open Biology 6: 150206 Akiyoshi B. (2016) The unconventional kinetoplastid kinetochore: from discovery toward functional understanding. Biochemical Society Transactions 44(5): 1201-1217 2014Akiyoshi B and Gull K. (2014) Discovery of unconventional kinetochores in kinetoplastids. Cell 156(6): 1247-582013Akiyoshi B and Gull K. (2013) Evolutionary cell biology of chromosome segregation: insights from trypanosomes. Open Biology 3(5): 130023 See full list in Pubmed This article was published on Monday 17 June 2024
