Publications and Preprints

2024

Ballmer D, Lou HJ, Ishii M, Turk BE, Akiyoshi B. An unconventional regulatory circuitry involving Aurora B controls anaphase onset and error-free chromosome segregation in trypanosomes. bioRxiv [Preprint]. 2024 Jan 20:2024.01.20.576407. doi: 10.1101/2024.01.20.576407

Ballmer D, Carter W, van Hooff JJE, Tromer EC, Ishii M, Ludzia P, Akiyoshi B# (2024) Kinetoplastid kinetochore proteins KKT14-KKT15 are divergent Bub1/BubR1-Bub3 proteins. Open Biology https://doi.org/10.1098/rsob.240025

Ballmer D, Akiyoshi B (2024). Dynamic localization of the chromosomal passenger complex in trypanosomes is controlled by the orphan kinesins KIN-A and KIN-B. elife https://doi.org/10.7554/eLife.93522

Ludzia P, Hayashi H, Robinson T, Akiyoshi B, Redfield C (2024). NMR study of the structure and dynamics of the BRCT domain from the kinetochore protein KKT4. Biomolecular NMR Assignment. https://doi.org/10.1007/s12104-024-10163-9

2022

Ishii M, Ludzia P, Marcianò G, Allen W, Nerusheva OO, Akiyoshi B (2022) Divergent polo boxes in KKT2 bind KKT1 to initiate the kinetochore assembly cascade in Trypanosoma brucei. Molecular Biology of the Cell (8A0J, 8A0K)

López-Escobar L, Hänisch B, Halliday C, Ishii M, Akiyoshi B, Dean S, Sunter JD, Wheeler RJ, Gull K. (2022) Stage-specific transcription activator ESB1 regulates monoallelic antigen expression in Trypanosoma brucei. Nature Microbiology

Ishii M and Akiyoshi B (2022) Targeted protein degradation using deGradFP in Trypanosoma brucei. Wellcome Open Research

Ishii M and Akiyoshi B (2022) Plasticity in centromere organization and kinetochore composition: lessons from diversity. Current Opinion in Cell Biology 

2021

Ludzia P and Akiyoshi B (2021) Illuminating the mechanism of monogenic antigen expression in trypanosomes. Nature Reviews Microbiology

Marcianò G, Ishii M, Nerusheva OO, and Akiyoshi B. (2021) Kinetoplastid kinetochore kinases KKT2 and KKT3 have unique centromere localization. Journal of Cell Biology (6TLX, 6LTY)

Tromer EC, Wemyss TA, Ludzia P, Waller RF, and Akiyoshi B (2021) Repurposing of synaptonemal complex proteins for kinetochores in Kinetoplastida. Open Biology 11: 210049

Ludzia P, Lowe ED, Marcianò G, Mohammed S, Redfield C, and Akiyoshi B (2021) Structural characterisation of KKT4, an unconventional microtubule-binding kinetochore protein. Structure doi.org/10.1016/j.str.2021.04.004 (6ZPM, 6ZPJ, 6ZPK)

2020

Akiyoshi B (2020, Preprint) Analysis of a Mad2 homolog in Trypanosoma brucei provides possible hints on the origin of the spindle checkpoint. bioRxiv

Ludzia P, Akiyoshi B, and Redfield C (2020). 1H, 13C and 15N resonance assignments for the microtubule-binding domain of the kinetoplastid kinetochore protein KKT4 from Trypanosoma brucei. Biomolecular NMR Assignments 14: 309–315

Ishii M and Akiyoshi B (2020) Characterization of unconventional kinetochore kinases KKT10 and KKT19 in Trypanosoma brucei. Journal of Cell Science 133(8): jcs240978

2019

Nerusheva OO, Ludzia P, and Akiyoshi B. (2019) Identification of four unconventional kinetoplastid kinetochore proteins KKT22–25 in Trypanosoma brucei. Open Biology 9: 190236

Akiyoshi B. (2019) Evolution: A Mosaic-type Centromere in an Early-Diverging Fungus. Current Biology 29: R1184-R1186

2018

Llauró A, Hayashi H, Bailey ME, Wilson A, Ludzia P, Asbury CL, and Akiyoshi B. (2018) The kinetoplastid kinetochore protein KKT4 is an unconventional microtubule tip-coupling protein. Journal of Cell Biology 217(11): 3886-3900

Hayashi H and Akiyoshi B. (2018). Degradation of cyclin B is critical for nuclear division in Trypanosoma brucei. Biology Open 23;7(3). pii: bio031609 

2017

Drinnenberg IA and Akiyoshi B (2017) Evolutionary Lessons from Species with Unique Kinetochores. In: Black B. (eds) Centromeres and Kinetochores. Progress in Molecular and Subcellular Biology, vol 56. Springer, Cham

2016

Nerusheva OO and Akiyoshi B. (2016) Divergent polo box domains underpin the unique kinetoplastid kinetochore. Open Biology 6: 150206 

Akiyoshi B. (2016) The unconventional kinetoplastid kinetochore: from discovery toward functional understanding. Biochemical Society Transactions 44(5): 1201-1217 

2014

Akiyoshi B and Gull K. (2014) Discovery of unconventional kinetochores in kinetoplastids. Cell 156(6): 1247-58

2013

Akiyoshi B and Gull K. (2013) Evolutionary cell biology of chromosome segregation: insights from trypanosomes. Open Biology 3(5): 130023 

See full list in Pubmed